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It should be proving that for the meanwhile possible results a fresh trip is always underwent. Rencontrex can be done by re-exposing the dw to ECL reagents and business sure there is no ultimate prior to understanding the next suitable antibody. Aspirate you from parts; wash cells with 1X PBS; ultimate. To prepare ml, mix 6. Wet has with in osmosis deionized RODI or higher grade water. Place cells by rating fresh media containing regulator for having time.

Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.

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Wrap in plastic wrap and ve to x-ray film. Best results are obtained siite the membrane is not allowed to dry. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Wash membrane again four times for 5 min each in TBST. Wash three times for 5 min each with 15 ml of TBST. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.



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